Ectoine and Hydroxyectoine from Halophilic Bacteria Isolated from Traditional Solar Saltern at Pejarakan Village, Buleleng Regency, Bali

The increased use of ectoine and hydroxyectoine in cosmetics pharmaceuticals has led to the increasing demand for these bioactive compounds. This study aims investigate production by halophilic bacteria isolated from traditional solar saltern at Pejarakan Village, Buleleng Regency, Province Bali, Indonesia. were grown brine sediment samples using Luria Bertani media. was determined inoculating MM63 media containing 12% w/v NaCl. Ectoine then extracted bacterial cells a mixture methanol/chloroform/water (10/5/4). Finally, ability excrete investigated osmotic downshock technique. results showed that total 88 have been which varied salt tolerances 0 27.5% w/v. Most (86 isolates) able grow media, suggesting potential producing hydroxyectoine. Further investigation 33 selected produce with levels 9.1 301.8 mg/L 3.9 122.1 mg/L, respectively. All also after process 9.8 277.5 14.3 122.7 best hydroxyectoine-producing closest homology relationship Salinivibrio costicola kushneri. Our found several promising candidates producers.